畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (2): 338-347.doi: 10.11843/j.issn.0366-6964.2018.02.013

• 营养与饲料 • 上一篇    下一篇

苜蓿不同留茬高度对低温胁迫的响应及抗寒能力评价

王伟1, 贾玉山1*, 格根图1, 降晓伟1, 尹强2   

  1. 1. 内蒙古农业大学草原与资源环境学院 农业部饲草栽培、加工与高效利用重点实验室, 呼和浩特 010018;
    2. 中国农业科学院草原研究所, 呼和浩特 010010
  • 收稿日期:2017-05-12 出版日期:2018-02-23 发布日期:2018-02-23
  • 通讯作者: 贾玉山,教授,主要从事饲草料加工与贮藏研究,E-mail:jys_nm@sina.com
  • 作者简介:王伟(1989-),男,内蒙古锡林郭勒盟人,博士生,主要从事饲草料加工与贮藏研究,E-mail:184105855@qq.com
  • 基金资助:

    国家自然科学基金面上项目(31572461)

Field Evaluation of the Response and Resistance to Low Temperature of Alfalfa with the Different Stubble Heights during Overwintering

WANG Wei1, JIA Yu-shan1*, GE Gen-tu1, JIANG Xiao-wei1, YIN Qiang2   

  1. 1. Key Laboratory of Forage Cultivation Processing and High Efficient Utilization of Ministry of Agriculture, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, China
  • Received:2017-05-12 Online:2018-02-23 Published:2018-02-23

摘要:

旨在探究末茬苜蓿不同留茬高度在越冬期间对低温胁迫的响应及抗寒性能力的强弱。本研究以“金皇后”(Medicago sative L. “Gold empress”)、“准格尔”(Medicago sative L. “Zhungeer”)、“WL323”(Medicago sative L. “WL323”)为试验材料,测定3个苜蓿品种的4种留茬高度(0、0~5、5~10、10~15 cm)在自然环境下根内可溶性糖(WSS)、可溶性蛋白(SP)、脯氨酸(Pro)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,综合评价各苜蓿品种及其末茬不同留茬高度的抗寒能力。结果表明:1)可溶性糖、脯氨酸、可溶性蛋白和过氧化氢酶活性随气温降低而升高,随气温升高而降低;超氧化物歧化酶活性和丙二醛含量在不同品种、不同留茬高度之间随气温变化规律不同。2)综合评价3个苜蓿品种抗寒能力强弱:准格尔 > 金皇后 > WL323,同一苜蓿品种末茬不同留茬高度抗寒能力强弱为A3 > A2 > A1 > A0。不同苜蓿品种抗寒能力不同,建议末茬留茬高度为10~15 cm。

Abstract:

The aim of this study was to investigate the effect of stubble height on the response to the low temperature and cold-resistance overwintering. Three alfalfa cultivars of "Gold empress", "Zhungeer" and "WL323" were selected as experimental materials. The alfalfa with 4 stubble heights(0, 0-5, 5-10, 10-15 cm)were measured for the water soluble sugar(WSS), proline(Pro), MDA, soluble protein(SP) contents, SOD and CAT activities in the roots under natural environment, the cold-resistance of different cultivars with different stubble heights were evaluated comprehensively. The results showed that:1) The WSS, Pro, SP contents and CAT activity showed opposite trend to temperature; the trend of SOD activity and MDA content of different cultivars and stubble heights showed different variation with the temperature variation. 2) The cold-resistance of different alfalfa cultivars were "Zhungeer" > "Gold empress" > "WL323", and the cold-resistance of the same alfalfa cultivar with different stubble heights were A3 > A2 > A1 > A0. The results indicate that the cold-resistance of different alfalfa cultivars are different, and the recommended stubble height is 10-15 cm.

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